Determination of saponification and iodine number

This is internationally accepted since the measurement is often run by a KOH solution in alcohol. Eventually, the solvent builds up in the extraction chamber and completely surrounds the sample.

If the solid fat content or crystal structure is important it may be necessary to carefully control the temperature and handling of the sample. If you have any questions about the chemistry of soap making please feel free to contact me! The percentage of lipid in the initial sample Msample can then be calculated: Chemical Techniques A number of chemical methods have been developed to provide information about the type of lipids present in edible fats and oils.

It deals with the structures and functions of cellular components such as proteins, carbohydrates, lipids, nucleic acids and other biomolecules. The smoke point is the temperature at which the sample begins to smoke when tested under specified conditions.

Thus, a low value of PV may represent either the initial or final stages of oxidation.

Iodine value

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The acid value is often a good measure of the break down of the triacylglycrols into free fatty acids, which has an adverse effect on the quality of many lipids.

Oxidation of unsaturated lipids can be minimized by adding antioxidants, or by flushing containers with nitrogen gas and avoiding exposure to heat and light. Special instruments are available to carry out solvent extraction at elevated temperatures and pressures.

The iodine value was If a bar of soap is too soft it will dissolve prematurely and become a mushy mess; so make sure that your soap has a certain level of hardness by combining hard oils with soft oils. In this case the aqueous phase would undergo further extractions using fresh solvent, then all the solvent fractions would be collected together and the lipid determined by weighing after evaporation of solvent.

By measuring the chemical shift spectra it is possible to determine the concentration of specific types of chemical groups present, which can be used to estimate the concentration of different types of lipids. Introduction In addition to their nutritional importance lipids are also used in foods because of their characteristic physicochemical properties, such as mouthfeel, flavor, texture and appearance.

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The ideal solvent for lipid extraction would completely extract all the lipid components from a food, while leaving all the other components behind. With a pipet add The thimble is placed in an extraction chamber, which is suspended above a flask containing the solvent and below a condenser.

What Is an Iodine Value?

A small amount of the lipid sample to be analyzed is spotted onto the TLC plate. This number usually tells you how much potassium hydroxide potash is needed instead of how much sodium hydroxide lye is needed.

Animal and vegetable fats and oils are mixtures of triglycerides.

process flow chart for a continous soap saponification plant

A TLC plate is coated with a suitable absorbing material and placed into an appropriate solvent. Thus different molecules can be separated on the basis of the strength of their interaction with the matrix.

Saponification value

The temperature at which the fat just starts to move downwards due to its weight is called the "slip point". The concentration of oil droplets in dilute food emulsions can be determined using light scattering techniques because the turbidity of an emulsion is directly proportional to the concentration of oil droplets present.

The fact that it behaves like a gas means that it can easily penetrate into a sample and extract the lipids, while the fact that it behaves like a fluid means that it can dissolve a large quantity of lipids especially at higher pressures.Determination of Saponification Number of Fats and Oils Elimination of the Blank.

William Rieman, III. Determination of Water in Aniline by Cloud Point Method. Determination of Iodine Numbers. Analytical Chemistry. Hiscox. 20 (7), pp – Saponification value (or "saponification number"/"Koettstorfer number", also referred to as "sap" for short) represents the number of milligrams of potassium hydroxide required to saponify 1g of fat under the conditions specified.

Peroxide Value by Titration

It is a measure of the average molecular weight (or chain length) of all the fatty acids present. As most of the mass of a fat/tri. The saponification value corresponds to the milligrams of potassium hydroxide (KOH) required to saponify the esters and neutralize the free acids in 1 g of a sample.

Nov 05,  · The saponification number is an indication of the average molecular weight of a fat and is determined by breaking it down into glycerol and a fatty acid salt by treatment with a strong alkali. The acid number indicates how much free fatty acid a fat contains and is estimated from the amount of alkali required to neutralize it.

Petroleum products -- Determination of saponification number -- Part 2: Potentiometric titration method This standard was last reviewed and confirmed in Therefore this version remains current. Saponification number formula is the amount of KOH consumed by 1g of fat.

Weight of KOH is the product of the normality of KOH, equivalent weight and volume of KOH in litres. The formula to find the Volume of KOH consumed by 1g fat is [Blank – test]ml.

Determination of saponification and iodine number
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